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DREG-55 mAb increases force-free affinity of L-selectin.
Jurkat cells were incubated with human PSGL-1-Fc or E-selectin-Fc in the presence of DREG-55 or -200 mAb and the fluorescence of the secondary anti-human IgG-FITC antibody was measured by flow cytometry. In untreated samples, only soluable ligand and secondary antibody but no DREG antibody was added. MFI is expressed relative to DREG-200 mAb samples. Mean ± S.D. of 3 to 4 experiments.
