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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: J Immunol. 2014 Jan 15;192(4):1796–1805. doi: 10.4049/jimmunol.1300087

Figure 2. Chemical inhibition of SGK1 abrogates neutrophil survival through an increase in apoptosis.

Figure 2

Human neutrophils were treated as indicated for 8 hours and percentage apoptosis scored by cytospin analysis. a) GSK650394 causes an increase in apoptosis rates and the 10 μM concentration significantly inhibits GM-CSF induced neutrophil survival (t-test, *p<0.05, n=5). b) Caspase inhibition prevents all neutrophil death (t-test, *p0.05, n=4). c) Neutrophil survival factors lower apoptosis rates at 8 hours, GSK650394 treatment abrogates survival caused by GM-CSF and DMOG but not that by LPS and ATPγs (*p<0.05, n=3).