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. Author manuscript; available in PMC: 2014 Feb 11.
Published in final edited form as: FEBS Lett. 2012 Oct 27;586(23):4208–4214. doi: 10.1016/j.febslet.2012.10.024

Figure 2.

Figure 2

Yeast projection width and length are inversely related to pheromone concentration. (A) Yeast cells after 3 hours exposure of α-factor (0, 3, 10, 30, 100 and 1000 nM) form morphologically distinct mating projections (scale bar = 5μm) (B) Schematic of projection length and width quantification. Projection length and width are determined by comparing cell images before and after exposure to a pheromone gradient. (C) Statistics of projection length and width normalized by pre-stimulation cell diameter are compared after exposure to high and low concentrations of α-factor (10 nM, blue bars, n=24; 30 nM, green bars, n=24; 1000 nM, red bars, n=24; there was a significant difference between the 10 nM and 1000 nM data by t-test, p < 0.001).