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. Author manuscript; available in PMC: 2015 Jan 7.
Published in final edited form as: Lab Chip. 2013 Nov 7;14(1):147–156. doi: 10.1039/c3lc51039e

Figure 2.

Figure 2

A. HER2 protein expression levels in breast cancer cell lines and healthy donor PBMCs tested by flow cytometry using the anti-HER2 antibody clone 9G6.10 (red lines) or IgG control (blue lines). Live cells were incubated with anti-HER2 antibody or matching IgG followed by secondary Alexa 488. Ten thousand events were acquired for each condition. X-axis shows logarithmic fluorescence intensity; Y-axis shows percent of max. B. Relative Mean Fluorescence Intensity (MFI) of HER2 protein assayed by flow cytometry; HER2 MFI was normalized using the IgG control MFI in each cell line. (SEM shown as error bar).