Figure 6. Dose-dependent inhibition of NO and TNF-α production by α-GalCer analogs 4d and 4e in LPS-stimulated BV2 microglial cells.

Cells were incubated for 16 h with LPS in the absence or presence of α-GalCer analogs 4d and 4e (5–10 µM), and the amounts of released NO and TNF-α were measured in supernatants. Treatment with α-GalCer analogs alone did not affect NO or TNF-α production. Bars indicate the mean ± S.E.M. of three independent experiments. *P<0.05; significantly different from LPS-treated microglial cells.