Figure 10. Biochemical properties of the methyltransferase activities exhibited by the recombinant PtrGXM proteins.

Recombinant PtrGXM proteins were incubated with ¹⁴C-labeled S-adenosylmethionine and the (GlcA)Xyl₄ acceptor, and the methyltransferase activity was determined by the transfer of the radiolabeled methyl group onto the acceptor. Error bars denote the se of three independent assays. (A) Time course of the transfer of the methyl group onto the (GlcA)Xyl₄ acceptor. (B) The methyltransferase activity is protein concentration-dependent. (C) Effect of temperature on the methyltransferase activity.