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. 2014 Feb 11;9(2):e88507. doi: 10.1371/journal.pone.0088507

Figure 9. 5-MTP blocks PMA-induced p300 HAT activity in SF-Fb.

Figure 9

A). SF-Fb were pretreated with 5-MTP at indicated concentrations for 30 min followed by PMA for 4 h. p300 HAT activity was measured. The error bars show mean ± SEM (n = 3). B). SF-Fb were treated with 5-MTP (10 µM) in the absence of PMA (left panel) or presence of PMA (right panel). Right panel also shows treatment of pFb with or without 5-MTP followed by PMA. Each error bar refers to mean ± SEM (n = 3). “NS” denotes statistically non-significant. C). SF-Fb were pretreated with 5-MTP (10 µM) for 30 min followed by PMA (100 nM) for 4 h. COX-2 proteins were analyzed with Western blotting. This Western blot is representative of two experiments with similar results. D). SF-Fb or pFb were treated with or without PMA (100 nM) for 4 h. p300 proteins were isolated by IP, washed and treated with 5-MTP (10 µM) for 30 min. HAT activity was measured. The error bars refer to mean ± SEM (n = 3). “NS” denotes statistically non-significant.