Figure 2. Thapsigargin-induced intacellular Ca²⁺ release and subsequent SOCE in DCs from gsk3^KI and gsk3^WT mice.

A. Representative original tracings showing [Ca²⁺]_i in Fura-2/AM loaded gsk3^WT (open circles) and gsk3^KI (closed triangels) DCs prior to and following removal of extracellular Ca²⁺, addition of SERCA inhibitor thapsigargin (1 µM) and readdition of extracellular Ca²⁺. B. Arithmetic means ± SEM (n = 44–59) of the peak (left) and slope (right) values of [Ca²⁺]_i increase upon Ca²⁺ release from intracellular stores (upper bars) and upon SOCE (lower bars) in gsk3^WT DCs (white bars) and gsk3^KI DCs (black bars). ***(p<0.001), unpaired t-test.