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A, Flies expressing PrP ubiquitously (da>PrP-M9) were fed with 17-DMAG at 0, 12, 24, 48, and 96 µg/ml during development. Analysis of PrP levels in 1 day-old flies by western blot showed no significant changes. Tubulin was used as loading control to normalize protein levels. B, Quantification of the triplicate experiment followed by analysis with ANOVA. No statistical differences were identified in these experiments.
