Table 1. Injections of TAL 3 mRNA and donor plasmid DNA in rat one-cell embryos.

Dose TALEN mRNA/DNA (ng/µl)	No. Injected eggs/No. Transferred eggs (% of survival)	Offspring (% of injected eggs)	No. NHEJ pups (% of offspring)	No. KI pups (% of offspring)	Targeting frequency (% of offspring)
40 (20+20)/5	225/171 (76)	32 (14)	4 (13)	1 (3)	16
20 (10+10)/5	293/228 (79)	27† (9)	5 (19)	0	19

^†One pup was born dead.

Two doses of TAL 3 mRNA were used (20+20 or 10+10 ng/µl of each TALEN). The egg survival rate is shown in percentage. NHEJ indicates the number of pups that had a gene disruption event in the sequence around pA476T. The percentages were calculated within each set of TALEN mRNA amount injected.