Fig. 2.

Identification of the Tnnt3 splicing isoforms in vastus lateralis and soleus of WT and FRG1 mice. A: frequency of the Tnnt3 isoforms cloned from vastus lateralis cDNA of in 13-wk-old WT (open bars) or FRG1 (solid bars) mice. Twenty-nine and thirty clones from two cDNA libraries representing Tnnt3 transcripts from WT and transgenic muscles, respectively, were full-length sequenced. Isoform identification numbers were assigned on the basis of the predicted protein length, and letters A or B were assigned on the basis of the presence of exon 16 or exon 17. B: frequency of the Tnnt3 isoforms cloned from soleus of 13-wk-old WT or FRG1 mice. Twenty clones from the WT and 21 from the transgenic cDNA libraries were full-length sequenced. A complete list of all the isoforms identified is shown in Table 1. C and D: The most abundant isoforms from WT and FRG1 vastus lateralis or soleus have been expressed as recombinant proteins, and their migration was compared with the fast skeletal troponin T pattern in muscle protein extracts by SDS-PAGE and immunoblotting.