Fig. 5.

l-NAME treatment impaired bone formation after WBF loading. A: ¹⁸F fluoride flow rate (K₁) was significantly decreased at day 1 in l-NAME-treated animals vs. vehicle. B: ¹⁸F fluoride incorporation rate (k₃) was significantly decreased at both days 1 and 3 in l-NAME-treated animals vs. vehicle. Representative images from dynamic histomorphometry 10 days after WBF loading in l-NAME-treated (C) and vehicle-treated (D) animals. Newly mineralized woven bone is labeled red. E: woven bone area was decreased 23% in l-NAME-treated animals. By microCT, woven bone volume was decreased 27% (F) and woven bone BMD was decreased 26% vs. vehicle (G). Mean ± standard deviation, n = 6, *P < 0.05 vs. vehicle.