Fig. 6.
Effects of perfusion of A2A receptor antagonist, ZM-241385, on the state-dependent discharge of VLPO sleep-active neurons. A: DR of 6 VLPO sleep-active neurons were quantified during baseline perfusion of aCSF and following a 10- to 15-min perfusion of ZM-241385 (50 μM in aCSF). For 4 cells, drug perfusion was initiated after 90–100 min of SD, and for 2 other cells drug was perfused during spontaneous sleep-wake occurring between ZT2 and ZT4. There was a significant decrease in waking DR (paired t-test, t = 2.58, df = 5, *P < 0.05) and NREM sleep DR (t = 3.46, df = 5, *P < 0.05) following ZM-241385 perfusion compared with baseline. B: perfusion of ZM-241385 had no effect on waking and NREM sleep DR in a group of 5 wake-active neurons recorded in the VLPO region.