Fig. 4.
DHPG enhances INaP when ICAN is blocked. A: voltage-ramps elicit the TTX-sensitive INaP in this example ET cell using a BAPTA-based recording pipette (see main text). Note that DHPG (30 μM) enhances INaP, whereas the DHPG-evoked ICAN is absent in this condition (compare with Figs. 1A and 2A). B and C: single cell (B) and group (C; n = 7) data subtraction plots show the DHPG-evoked enhancement of INaP. C includes the I–V profile of INaP obtained with the standard recording solution (Control-No BAPTA). Note that INaP magnitude is larger at depolarized potentials (−45 to −40 mV) compared with that obtained with a BAPTA-based internal solution.