Figure 3.

A CaN increase induced by 10 CSs is required for consolidation of the fear extinction memory. A, Animals with fully consolidated fear memory received microinfusions of ASO or Scr ODN sequences in the BLA (see Fig. 2) 2.5 h before the presentation of 1 CS (n = 10 per group) or 10 CSs (Scr ODN, n = 10; ASO, n = 8). Four days later, all groups were tested for long-term fear or extinction memory retention by the presentation of one CS. *p < 0.005. B, Animals with fully consolidated fear memory were microinfused with ASO (n = 5) or Scr ODN (n = 4) 2.5 h before 10 CS presentations. One hour after the 10 CS presentations, they were killed and the BLA cytoplasmic extract was obtained. A representative result of the Western blot analysis along with the quantification of CaN levels in the BLA is shown. Data are presented as means ± SEM. *p < 0.05.