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. 2014 Feb 12;34(7):2444–2450. doi: 10.1523/JNEUROSCI.4085-12.2014

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Copyright © 2014 the authors 0270-6474/14/342444-07$15.00/0

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Figure 1. — Site-specific Bdnf knockdown and TrkB dominant-negative inhibition with lentiviral vector approaches. A, Qualitative image showing Bdnf in situ hybridization of the amygdala after LV-GFP injection or (B) after LV-Cre injection. C, Qualitative image showing Cre-recombinase in situ hybridization of the amygdala after LV-Cre injection. D, Lentiviral infection with LV-GFP into amygdala (4×). E, Lentiviral infection with LV-TrkB.t1 into amygdala (4×), visualized with an anti-HA antibody. F, LV-TrkB.t1 visualized with an anti-HA antibody and revealed by 3,3′ diaminobenzidine (DAB) (20×). G, Infusion maps, drawn upon Paxinos and Franklin (2001) Mouse Atlas Diagrams, for the maximal (left) versus minimal (right) extent of LV-Trkb.t1 viral infusions. The actual infusions were bilateral, with no noticeable difference in left versus right infusion volume.