Table 1.
RSD increased CD11b+ splenocyte proinflammatory gene expression after LPS stimulation
| Gene | LPS | WT |
eIL-1R1kd |
||
|---|---|---|---|---|---|
| CON | RSD | CON | RSD | ||
| IL-1β | |||||
| − | 1.05 ± 0.16 | 4.30 ± 1.19# | 0.85 ± 0.17 | 7.06 ± 2.49# | |
| + | 4.83 ± 0.89* | 24.16 ± 6.70*,‡ | 4.60 ± 1.13* | 25.72 ± 6.19*,‡ | |
| TNF-α | |||||
| − | 1.04 ± 0.04 | 4.89 ± 1.49# | 0.80 ± 0.17 | 5.91 ± 1.93# | |
| + | 8.81 ± 0.65* | 45.78 ± 10.45*,‡ | 8.53 ± 2.84* | 47.82 ± 14.40*,‡ | |
| IL-6 | |||||
| − | 1.00 ± 0.05 | 4.86 ± 1.09# | 0.99 ± 0.02 | 2.53 ± 0.52# | |
| + | 6.42 ± 1.21* | 43.11 ± 13.48*,‡ | 8.18 ± 1.88* | 26.24 ± 6.10*,‡ | |
WT and eIL-1R1KD FVB mice were subjected to RSD or left undisturbed as CON mice. Enriched CD11b+ splenocytes were stimulated with saline (−) or LPS (+). Relative mRNA expression of IL-1β, TNF-α, and IL-6 in LPS-stimulated CD11b+ splenocytes is shown. Values represent average ± SEM.
*p < 0.05, significantly different from WT–CON(−);
#p < 0.10, different from WT–CON(−);
‡p < 0.05, significantly different from WT–CON(+) and WT–RSD(−).