Figure 3.

Treadmill training attenuates inflammation in adipose tissue from obese mice. A, Increasing physical activity reduces body weight gain in db/db mice. B, The weight of the epididymal fat pad was significantly reduced by treadmill training in db/db mice. C, H&E staining of epididymal fat from the indicated groups. D, The area of individual adipocytes was smaller in TT db/db mice relative to SED db/db mice. E, Levels of IL1β in the epididymal fat pad were elevated in SED db/db mice but not in TT db/db mice. F, Serum IL1β was also reduced by exercise in db/db mice. G, Western blotting of epididymal fat pad protein extracts revealed that exercise attenuates the accumulation of proinflammatory proteins in adipose tissue. Graph at right depicts band intensities for the indicated groups. For all graphs, *p < 0.05 relative to WT SED mice after 2 × 2 ANOVA with Bonferroni's-corrected post hoc t tests. Error bars represent the SEM.