Figure 7.

Evoked GABAergic transmission and the number of GABAergic synapses were reduced in the mPFC of Emx1-Mecp2 mutant mice. A, Monosynaptic IPSCs recorded from control and mutant neurons evoked by local stimulation. B, The input–output curve of IPSCs for control (n = 13 from 3 mice) and mutant (n = 14 from 3 mice) neurons. C, Paired pulse responses for IPSCs from control and mutant neurons. The averaged IPSCs from control and mutant neurons were normalized to the peak of the first IPSC. The paired pulse ratio at 100 ms interval was 0.89 ± 0.02 (n = 10 from 3 mice) for mutant neurons, and 0.85 ± 0.03 (n = 11 from 3 mice) for control neurons (p = 0.14). D, Double immunostaining of VGAT (red) and NeuN (green) in the mPFC of control and mutant mice at P18 (top) and 7 weeks of age (bottom). Scale bar, 10 μm. E, Numbers of VGAT puncta per 1000 μm³ volume in layer 5 of mPFC of mutant and control mice at P18–P19 and 7–8 weeks of age. Data were obtained from four pairs of mice at P18–P19 and 7–8 weeks of age, respectively; three measurements were performed for each mouse. P values were obtained with Mann–Whitney test.