Fig. 1.

Gonadotropin-releasing hormone immunofluorescence (IF-GnRH) and enhanced green fluorescent protein (EGFP)-GnRH (TG-GnRH) neurons on coronal sections of the preoptic area (POA) in P0, P5 and P52 males. More IF-GnRH neurons (red) were observed compared to their respective TG-GnRH cells (green) in the POA of P0 (a) and P5 (b) males. Number of TG-GnRH neurons was comparable to the IF-GnRH neurons in P52 (c) male, as most TG-GnRH cells were colocalized with GnRH immunofluorescence (yellow) in POA. High-magnification images of TG-GnRH neurons (green) double-labeled with IF-GnRH (red) and merged images (yellow) in P0 (d–f) and P52 (g–i) males. Intensity of the IF-GnRH on TG-GnRH neurons in P0 male appeared less compared to the P52 male. Bars 100 μm (a–c) and 10 μm (d–i)