Table 3. Mean shrinkage rates of GDP MTs.
ATP (μM) | Fast (plus) end (nm s−1±s.e.m. (n)) | Slow (minus) end (nm s−1±s.e.m. (n)) | |
---|---|---|---|
A-lattice (Mal3FL) | 0 | 10.68±0.30 (5) | 2.78±0.42 (5) |
B-lattice | 0 | 2.68±0.73 (5) | 0.34±0.13 (5) |
B-lattice | 1 | 55.14±5.12 (5) | 7.06±2.82 (5) |
Mean shrinkage rates of GDP MTs held in a rigor kinesin clamp were compared before and after the release of the clamp by addition of 1 μM ATP. The shrinkage at the plus end was 29 times faster in the A-lattice enriched microtubules and significantly different from the B-lattice (t-test, P<0.0001). The slow ends of A-lattice-enriched MTs also shrank significantly faster than in the B-lattice MTs by 8 times (t-test with Welch’s correction for unequal variances, P=0.0054).