Figure 1.
Results of immunohistochemical staining. A-C: Immunohistochemistry for (A) Skp2, (B) Cks1, and (C) Ki67 proteins showed that stains were mainly expressed in the nuclei of cancer cells; D: The staining pattern of p27kip1 was somewhat heterogeneous. The pattern of nuclear staining was mainly noticed, and some of them were expressed as diffuse cytoplasmic staining. The staining intensity of Skp2, Cks1, and p27kip1 was classified as follows: 0, no staining; 1, weak; 2, moderate; 3, strong. The staining pattern of Ki-67 was nuclear and classified as positive or negative. At least 20 high-power fields will be randomly chosen, and 2000 cells will be always counted. Skp2: S-phase kinase-associated protein 2; Cks1: Cyclin-dependent kinases regulatory subunit 1.