Table 3.

Detection rates of Campylobacter concisus in fecal samples by two polymerase chain reaction methods

	Campylobacter genus PCR	Nested PCR
CD (n = 54)	19%	65%
Healthy controls (n = 33)	12%	33%
Non-IBD controls (n = 27)	0	37%

An example showing that different polymerase chain reaction (PCR) methods affect the detection of Campylobacter concisus (C. concisus) in intestinal samples. Data included in this table were from reference 18. Primers C412F and C1288R were used in campylobacter genus PCR. In nested PCR, PCR products amplified by primers C412F and C1288R were amplified again using C. concisus specific primers Concisus F and Concisus R. The nest PCR detected a significantly higher intestinal prevalence of C. concisus in patients with Crohn’s disease (CD) compared to both healthy controls and non-inflammatory bowel disease (IBD) controls. The genus PCR detected a significantly higher intestinal prevalence of C. concisus in patients with CD compared to non-IBD controls, but not healthy controls.