Figure 2.

Vard inhibits cell proliferation and apoptosis in the colon mucosa. (a) IHC staining of Ki-67 (proliferative cells) and TUNEL (apoptotic cells, red arrows) in colon mucosa of WT mice treated with Vard for 7 days. Control mice were injected with vehicle. Scale bars, 20 μm. (b) Quantification of Ki-67-positive cells and (c) TUNEL-positive cells from the stained tissue sections from the proximal (prox), middle (mid) and distal colons from WT and PKG2 KO mice. Solid lines show Vard treated, and dashed lines show Controls. Numbers are presented as nuclei per crypt. *P<0.05, **P<0.01, ***P<0.001