Skip to main content
. 2013 Nov 22;21(3):427–437. doi: 10.1038/cdd.2013.163

Figure 4.

Figure 4

DUSP10 is a PKG2 effector in the colon epithelium. (a) RT-PCR detecting mRNA levels of DUSP10 and PKG2 in colon mucosa of three WT/KO siblings. (b) WT/KO mice colon mucosa explants were treated with (+) or without (−) 8Br-cGMP. RT-PCR showing mRNA levels of DUSP10 (upper panel) and western blots showing phospho-JNK T183/Y185 (lower panel). HPRT1 and total JNK were used as loading controls respectively. (c) RT-PCR showing expression levels of DUSP10 in mucosa from ileum (I) and colon (C) of CD-1 mice treated with Vard or with vehicle (Ctrl). (d and e) PKG2 KO mice were treated with the JNK inhibitor SP600125 (KO+SP, dark bars) or vehicle (KO, open bars) and the colon was processed for quantification of (d) proliferative cells (Ki-67), apoptotic cells (TUNEL) and (e) mature goblet and enteroendocrine cells (CgA). Numbers are presented as a percentage of the respective mean in WT mice. *P<0.05