Figure 3.

MiR-25 is a major contributor to doxorubicin resistance. MTMEC cells were transfected with lentiviral vectors harboring genomic sequences of individual miRs to generate MTMEC-miR-106b, MTMEC-miR-93 and MTMEC-miR-25 cells (cells transfected with the empty vector, MTMEC-ev, were used as control). (a) Expression of miRs was determined by reverse transcription and real-time PCR using TaqMan probes and was normalized to the expression of U6 RNA. Cells were treated for 24 h with 60 ng/ml doxorubicin and (b) drug-resistant clones were stained with crystal violet after 3 weeks and (c) counted. (d) Percentage of cells incorporating EdU by fluorescence microscopy. (e) EdU incorporation determined by fluorescence detection. Numerical data represent the average of three independent experiments±S.D. (*P<0.05). Pictorial data show a representative of at least three independent replicates