Figure 5.

The miR-106b∼25 cluster regulates migration and invasion. Motility was monitored by the wound-healing assay (a) and quantified using trans-well assays (b), whereas invasion was quantified in the same way but using Matrigel-coated trans-well chambers (c). (a) Wounds were inflicted with a needle tip on confluent layer of cells (top panels) and monitored by microscopy 18 h later (bottom panels). The white lines define the areas lacking cells at the beginning of the experiment. (b and c) After 72 h, cells in the transwell were stained with crystal violet (upper panels) and then the dye was solubilized and quantified at 592 nm. Numerical data represent the average±S.D. of at least three experiments (*P<0.05). Pictorial data show a representative of at least two replicates