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. 2014 Feb 12;4:4075. doi: 10.1038/srep04075

Figure 2.

Figure 2

(A) Confirmation of the expression of intrinsic biomarkers-EGFR, CD44 and TGFβRII in 10 (a) and 15 μg (b) of total protein extracted from cultured MDA-MB-231 by western blot. Total proteins from MDA-MB-231 cells were electrophoresed under same experimental conditions and probed for β-actin, CD44 and TGFβRII proteins using respective primary antibodies followed by corresponding secondary antibodies. EGFR was detected by electrophoresing total proteins in SDS free conditions followed by probing using corresponding primary and secondary antibodies. (B) Cell viability study using Cell Counting Kit-8 on MDA-MB-231 cells exposed to pure AuNP, antibody-bioconjugated (w ab) and non bioconjugated (w/o ab) SERS nanotags (MGITC, Cy5 and Rh6G) after 24 hours.