Figure 4.

In vivo multiplex detection in xenograft tumor: (A):- Image of a tumor bearing mouse from test group used in experiment.(B):-SERS spectra from tumor site in a representative control mice showing the peaks at 1120,1175 and 1650 cm⁻¹ from non bioconjugated SERS nanotags corresponding to Cy5, MGITC and Rh6G respectively. Signal intensity is not detectable 6 hours after injection and nanotags gets cleared fast from the body due to the lack of specific binding. (C):- SERS spectra from tumor site in a representative test mice showing the peaks at 1120, 1175 and 1650 cm⁻¹ from the bioconjugated Cy5, MGITC and Rh6G nanotags bound to TGFβRII, CD44 and EGFR biomarker respectively. Due to active targeting, multiplex SERS spectra is observed up to 48 hours followed by the clearance of nanotags from the mouse body by ~72 hours.