Fig. 5.

PDGF BB mediates migration by reactive oxygen species (ROS) generation. A: quiescent metanephric mesenchymal cells were loaded with 5-(and-6-) chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (DCF; 10 μM) for 30 min before treatment with PDGF BB (20 ng/ml). Graph depicts the intensity (integrated densities) of fluorescence. ***P < 0.001 by 1-way ANOVA and Tukey's post hoc analysis. B: hydrogen peroxide induces migration in metanephric mesenchymal cells. Quiescent monolayers were wounded and treated with 1–10 μM H₂O₂. Migration was assessed as described. C: tiron inhibits PDGF BB-induced migration. Migration was assessed as described. *P < 0.05 by 1-way ANOVA and Tukey's post hoc analysis. D: effect of N-acetyl-l-cysteine (NAC) on PDGF BB-induced migration. Cells were pretreated with 2.5–20 mM NAC, and migration was assessed as described. E: effect of diphenyleneiodonium (DPI) on PDGF BB-induced migration was measured in metanephric mesenchymal cells. Monolayers were pretreated with 0.1–20 μM DPI. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. PDGF BB-treated group by 1-way ANOVA and Tukey's post hoc analysis.