Fig. 2.

Mir-194 interacts with 3′UTR of ITSN1. A: putative anneal site of mature human miR-194 to the human ITSN1 to 3′UTR. The bold fronts indicate the seed sequence. B: expression of miR-194 mimic decreases while expression of miR-194 inhibitor increases the activity of luciferase report gene in HEK293T cells transfected with a construct containing the ITSN-3′UTR. Scrambled nucleotide sequence (nt-control) was used as a negative control. C: bar graph showing that miR-194 mimic did not affect the activity of luciferase report gene in the cells transfected with mutant ITSN1-3′UTR (mut 3′UTR) in which the seed sequence for mir-194 was deleted. D: bar graph showing that miR-205 mimic did not affect the activity of luciferase reporter gene in the cells transfected with ITSN1-3′UTR.