Fig. 4.

miR-194 regulates the expression of ITSN1. A: expression of miR-194 decreases the expression of ITSN1 expression but not the expression of green fluorescent protein (GFP) in HEK293T cells transfected with flag-tagged ITSN1-3′UTR cloned into pTracerCMV vector which contains GFP driven by a different promoter. A bar graph demonstrates the normalized expression of ITSN1 at 0, 24, and 48 h after the transfection (bottom). B: mir-194 failed to inhibit 3′UTR-free ITSN1 expression. A Western blot shows the effect of miR-194 on the expression of ITSN1 in the cells transfected with flag-tagged and 3′UTR-free-ITSN1 cloned into pTracerCMV vector. The results are summarized in a bar graph (bottom).