Fig. 5.

Expression of miR-194 increases ROMK channel activity. A: whole cell recordings show the effect of expression of ITSN1 and miR-194 on the Ba²⁺-sensitive K currents. The experiments were performed 24 h after transfection with the perforated whole cell recording at the voltage from −100 to 60 mV at a 20-mV step. B: bar graph summarizes the above data from 6 such experiments at −100 mV. The bath and pipette solutions contained 140 mM KCl, 1.8 mM MgCl₂, 1.8 mM CaCl₂, and 10 mM HEPES (pH 7.4). The HEK293 T cells were transfected with GFP-tagged ROMK1 and pre-miR-194 which was cloned into pSilencerCMV vector. The HEK293T cells were transfected with GFP-ROMK1 (1 μg) + 1 μg negative control nucleotides (nt), 1 μg GFP-ROMK1 + 0.5 μg miR-194 mimic + 0.5 μg nt, 1 μg GFP-ROMK1 + 0.5 μg miR-194 + 0.5 μg ITSN1 for 24 h.