Fig. 6.

Mir-194 increases ROMK expression in the plasma membrane. HEK293T cells were transfected with GFP-tagged ROMK1 with control nt, pre-miR-194, or pre-miR-194+ITSN1 for 24 h. The biotin-labeled ROMK1 was immunoprecipitated with neutravidin beads and detected with GFP (ROMK) antibody. The surface expression of ROMK1 was normalized by calculating the ratio between the surface and total expression of ROMK1. The HEK293T cells were transfected with GFP-ROMK1 (1 μg) + 1 μg negative control nucleotides (nt), 1 μg GFP-ROMK1 + 0.5 μg miR-194 mimic + 0.5 μg nt, 1 μg GFP-ROMK1 + 0.5 μg miR-194 + 0.5 μg ITSN1 for 24 h.