Figure 6.

Effect of BaTX-I, BaTX-II, and BaPLA₂ on superoxide production by J774A.1 macrophages. 2 × 10⁵ macrophages were incubated with RPMI, 0.1% NBT, 10 uL of PMA (500 ng/mL) (positive control) or toxins (6 µg/mL) for 60 minutes at 37°C in a humidified atmosphere of 5% CO₂ for the formation of formazan crystals resulting from the reduction of NBT by superoxide. The crystals were solubilized and the absorbance of the supernatant was determined at 620 nm in a spectrophotometer. Data were expressed as O.D. Values represent the mean ± S.E.M. from 3 independent experiments. *P ≤ 0.05 compared with control (ANOVA).