Figure 8.

Effect of BaTX-I, BaTX-II, and BaPLA₂ on J774A.1 macrophage lysosomal volume. 2 × 10⁵ macrophages were incubated with the toxins (6 µg/mL) or RPMI (control) for 15, 30, and 60 minutes at 37°C in a humidified atmosphere of 5% CO₂. After that, the macrophages were incubated with 0.04% neutral red. The incorporated dye was solubilized and the absorbance of the supernatant was determined at 505 nm in a spectrophotometer. Values represent the mean ± S.E.M. from 3 independent experiments. *P ≤ 0.05 compared with control (ANOVA).