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. 2014 Jan 9;53(4):682–689. doi: 10.1021/bi401193w

Figure 4.

Figure 4

Transposon excision and in vitro transposition. (a) Schematic of the plasmid-based cleavage assays. The transposon donor plasmids (5.6 kb) contain a kanamycin resistance gene flanked by the inverted repeats (black triangles) of either Mos1 or Mboumar-9 (Mbo9). The expected products and their size (in kilobases) are indicated. (b) Agarose gel of the products of cleavage of the Mboumar-9 plasmid by Mboumar-9 (lanes 4–7) and Mos1 transposase (lanes 8–11). (c) Agarose gel of the products of cleavage of the Mos1 plasmid by Mos1 (lanes 4–7) and Mboumar-9 transposase (lanes 8–11). The control (lane 2) contained Mboumar-9 plasmid linearized with XbaI and digested with SacI, which cleaves outside the IRs. (d) Efficiencies of in vitro transposition reactions performed using Mboumar-9 or Mos1 transposase and donor plasmids containing either the Mboumar-9 or Mos1 inverted repeat (IR). Nine repeats were performed for experiments in which transposase acted on their own repeats, and cross-reactivity experiments were performed in triplicate.