Table 1.

Differentially expressed intracellular proteins (by twofold or more), as identified by liquid chromatography-tandem mass spectrometry between voriconazole susceptible, susceptible dose-dependent, and voriconazole resistant strains

Spot	Protein	Molecular mass (Dalton)	pI	Fold change (R/S)a
25	C1-tetrahydrofolate synthase	102,203	5.98	3.09
27	Formyltetrahydrofolate synthetase (FTHFS)	102,203	5.98	2.06
79	ACO1 aconitate hydratase aconitase	85,429	6.78	2.53
90	Potassium efflux protein KefA	73,694	5.41	2.05
115	Sphingolipid long-chain base sensory protein	40,387	5.54	2.27
116	TKL1 transketolase	73,704	6.01	2.50
127	Heat shock protein 70	112,540	7.87	3.5
189				2.15
540				2.13
154	Acetolactate synthase	73,300	8.55	2.41
202	LEU4 2-isopropylmalalate synthase	67,290	5.52	2.51
218	Acetyl-CoA hydrolase/transferase N-terminal domain	58,541	6.16	2.38
228	Phosphoribosylaminoimidazole carboxylase	62,672	6.95	4.42
238	Pyruvate kinase	55,563	6.25	2.66
263	Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes	61,726	5.59	3.94
295	Aldehyde dehydrogenase family	55,937	5.09	2.03
304	SES1 seryl-transcription RNA synthetase	52,775	5.8	2.19
319	Iinosine monophosphate dehydrogenase	56,969	6.69	2.86
396	Serine hydroxymethyltransferase	52,271	6.74	3.1
397				3.6
411	GDP dissociation inhibitor	50,582	5.66	3.02
476	Protein with specific affinity for G4 quadruplex nucleic acids	42,134	8.61	2.33
504	Isocitrate dehydrogenase	46,728	5.23	2.52
505	spP36046 Saccharomyces cerevisiae YKL195w	44,592	4.45	2.78
507	Chromosome segregation adenosine triphosphatases (ATPases)	55,271	8.81	14.1
535	Malate dehydrogenases (MDH) glycosomal and mitochondrial	39,024	6.15	2.42
550	Cyclophilin_ABH_like	41,620	5.61	2.2
560	Aspartate/tyrosine/aromatic aminotransferase	45,608	7.2	2.73
576	Quinone reductase and related Zn-dependent oxidoreductases	40,823	6.01	2.24
603	Branched-chain aminotransferase	41,550	5.82	2.58
619	Highly similar to S. cerevisiae YBR249c ARO4	38,617	6.51	2.24
636	RPC40 DNA-directed RNA polymerase I	37,577	5.22	2.05
645	S. cerevisiae YGR080w	36,175	5.02	3.49
777	Peptidase_S8 (serine proteinase)	50,008	5.75	2.25
107	Glycerol-3-phosphate dehydrogenase	43,961	5.85	−2.82
142	TKL1 transketolase	73,704	6.01	−3.57
321	F0F1 ATP synthase	58,485	8.99	−2.46
454	Effector domain of the CAP family of transcription factors	44,936	5.92	−2.14
570	Acetyl-CoA hydrolase			−2.53
609	Oxidoreductases	46,710	5.76	−2.4
613				−3.75
615	Malate dehydrogenases glycosomal and mitochondrial	40,487	9.18	−2.14
625	Phosphoglycerate kinase	44,590	6.37	−8.90
628	Arginase	35,061	5.27	−3.13
633	Highly similar to spP53252 S. cerevisiae YGR086c	35,129	4.68	−3.03
723	Uncharacterized enzymes related to aldose 1-epimerase	33,397	5.06	−3.15
774				−2.07
909	Hypothetical protein CAGL0I00616g	2,183	5.37	−9.09
912	ATP synthase D chain, mitochondrial (ATP5H)	19,918	6.64	−2.73

^aExpression ration of voriconazole-resistant (R) over voriconazole-susceptible (S) strains. The minus sign (−) indicates decreased protein expression of voriconazole-resistant strains in comparison with voriconazole-susceptible strains.