Table 3.
Differentially expressed membrane proteins (by twofold or more), as identified by liquid chromatography-tandem mass spectrometry between voriconazole susceptible, susceptible dose-dependent, and voriconazole resistant strains
| Spot | Protein | Molecular mass (Dalton) | pI | Fold change (R/S)a |
|---|---|---|---|---|
| 12, 314 | Enolase | 46,710 | 5.76 | 2.69, 2.56 |
| 132, 169 | Hsp70 protein | 6,635 | 5.32 | 2.18, 2.72 |
| 195, 379 | Pyruvate kinase (PK) | 54,572 | 8.26 | 2.13, 2.21 |
| 244 | Cysteine synthase | 55,388 | 5.51 | 2.34 |
| 255, 291 | Pyruvate decarboxylase | 61,726 | 5.59 | 2.12, 2.31 |
| 276 | Pyrophosphate-requiring enzymes | 46,993 | 4.46 | 3.66 |
| 284 | WD40 domain adaptor/regulatory modules in signal transduction | 46,504 | 4.44 | 2.34 |
| 457 | Phosphoglycerate kinase (PGK) | 44,590 | 6.37 | 2.77 |
| 50 | Heat shock protein | 80,983 | 4.82 | −2.43 |
| 119, 149 | Hsp70 protein | 69,469 | 4.96 | −2.43, −4.09 |
| 153, 226 | −2.18, −2.74 | |||
| 357, 552 | −2.54, −3.88 | |||
| 138, 174 | −28.0, −2.37 | |||
| 172 | −3.35 | |||
| 175 | Saccharomyces cerevisiae YLR259c Heat shock protein | 60,351 | 5.14 | −4.77 |
| 229 | Hexokinase | 53,772 | 5.23 | −2.39 |
| 260 | Aldehyde dehydrogenase family | 56,131 | 6.07 | −2.11 |
| 292 | F1 adenosine triphosphate (ATP) synthase beta subunit, nucleotide-binding domain | 54,176 | 5.14 | −2.68 |
| 298 | Nicotinamide adenine dinucleotide phosphate -glutamate dehydrogenase | 49,711 | 5.58 | −3.17 |
| 360 | SAM1 S-adenosylmethionine synthetase | 41,700 | 5.10 | −2.19 |
| 398 | ATPase alpha2,Na/K | 116,305 | 5.41 | −2.56 |
| 462 | N terminal of the Stm1 protein | 29,791 | 9.65 | −6.48 |
| 465 | Adenosine kinase (AK) | 36,250 | 5.23 | −2.05 |
| 548 | Exo-beta-1,3-glucanase | 33,667 | 4.41 | −2.50 |
| 557 | Elongation factor 1 beta (EF1B) guanine nucleotide exchange domain | 22,903 | 4.33 | −3.57 |
| 560 | Predicted epimerase, PhzC/PhzF homolog | 32,286 | 4.98 | −2.35 |
| 578 | Phosphoglycerate mutase 1 | 27,468 | 5.48 | −11.1 |
| 597 | Phosphoglycerate kinase | 18,458 | 7.85 | −2.51 |
| 602 | Mitochondrial ribosomal protein MRP8 | 24,160 | 4.73 | −2.12 |
| 616 | Ribosome antiassociation factor IF6 | 26,367 | 4.52 | −2.62 |
| 629 | TrpR binding protein WrbA | 29,728 | 6.54 | −2.08 |
| 632 | Alcohol dehydrogenase GroES-like domain | 36,721 | 6.21 | −2.25 |
| 645 | Type 1 glutamine amidotransferase (GATase1) | 25,479 | 5.16 | −4.45 |
| 658 | Phosphoglycerate kinase (PGK) | 44,590 | 6.37 | −2,15 |
| 715 | Chain A, yeast Cu, Zn enzyme superoxide dismutase | 15,714 | 5.63 | −7.19 |
a
Expression ration of voriconazole-resistant (R) over voriconazole-susceptible (S) strains. The minus sign (−) indicates decreased protein expression of voriconazole-resistant strains in comparison with voriconazole-susceptible strains.