Figure 3.

Multiple sequence alignment of FSA and other GH13_5, GH13_6, and GH13_7 α-amylases. Dark and grey backgrounds were adopted to highlight the positions of identical and similar residues, respectively. The positions of the amino acids coordinating the zinc ion are marked as ■; those coordinating calcium are marked as *; the mutated residues and FYW region are marked as a red box; the residues conserved in GH13_7 sequences are marked as green; residues conserved in Flavobacteriaceae amylases are marked as red; and residues conserved in both GH13_6 and GH13_7 sequences are marked as blue. FSA, Flavobacteriaceae Sinomicrobium α-amylase; GH13, glycosyl hydrolase family 13.