Figure 4.

Uptake of 30 nM [³H]pentamidine by aqp2 null and wild-type control cells. Cells of T. b. brucei strain 2T1 (closed symbols) or the derived aqp2 null strain (open symbols) were incubated with [³H]pentamidine for 90 s in the presence or absence of various concentrations of unlabelled pentamidine (circles) or propamidine (squares). The incubation was stopped by the addition of 1 mL ice-cold 1 mM pentamidine and immediate centrifugation through oil. The experiment shown is representative of four identical but independent experiments, each performed in triplicate and showing virtually identical outcomes. Data points are mean ± SEM of triplicates.