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. 2013 Nov 13;69(3):651–663. doi: 10.1093/jac/dkt442

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© The Author 2013. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effect of expression of T. brucei aquaporins on drug susceptibility of Leishmania mexicana. TbAQP2 and TbAQP3 were expressed in promastigotes using the pNUS vector. Two independent clones of each resulting cell line and the promastigotes transfected with the ‘empty’ pNUS vector were tested for sensitivity to (a) pentamidine, (b) Cymelarsan, (c) diminazene and (d) amphotericin B using the Alamar blue fluorimetric assay. Bars are means of three to eight independent determinations; error bars are SEM. ***P < 0.001 by one-way ANOVA with Tukey's correction (GraphPad Prism 5.0). EV, empty-vector control.