Figure 8.
Expression of synthetic aquaporin constructs in bloodstream forms of the aqp2/aqp3 double-null line. EC50 values were determined for (a) pentamidine, (b) Cymelarsan, (c) diminazene and (d) phenylarsine oxide. Expression was induced by the addition of 1 μg/mL tetracycline 24 h before setting up the plates for the assay, followed by 48 h of incubation of the cells in the presence of a doubling dilution of the test compound followed by a further 24 h of incubation with the dye before fluorescence was determined. All data are the mean of at least three independent determinations; error bars are SEM. Statistical significance with aqp2/aqp3 null was determined using a one-way ANOVA with Tukey's correction (GraphPad Prism 5.0); **P < 0.02; ***P < 0.01. In the Cymelarsan panel (b), it is also indicated that Groups 4 and 5 are significantly different from Group 3.