Table 1.

Overview of trypanosome strains used in this study

Strain	Subspecies	Susceptibilitya		Adapted from	Resistance induction	Infectivity		Transport activity			References
		pentamidine	arsenical			rodents	tsetse	P2	HAPT1	LAPT1
Lister 427	T. b. brucei	+++	+++	NA	NA	√	√b	√	√	√	14,16,17
2T1	T. b. brucei	+++	+++	Lister 427	NA	unkn	unkn	√	√	√	31,20
aqp2/aqp3 null	T. b. brucei	+/−	+	2T1	NA	unkn	unkn	√	NP	√	20
TbAT1-KO	T. b. brucei	++	++	Lister 427	TGD	√	unkn	NP	√	√	14
B48	T. b. brucei	−	+/−	TbAT1-KO	in vitro, pentamidine	√	unkn	NP	NP	√	16
P1000	T. b. brucei	−−−	+/−	B48	in vitro, pentamidine	unkn	unkn	NP	NP	√	—
STIB 247	T. b. brucei	+++	+++	NA	NA	√	√	√	√	√	33,57,52
STIB 247MR	T. b. brucei	+/−	−	STIB 247	in vivo, Cymelarsan	√	√	NP	NP	√	33,57,52
STIB 386	T. b. gambiense	+++	++	NA	NA	√	√	√	√	√	33,57,52
STIB 386MR	T. b. gambiense	+/−	−	STIB 386	in vivo, Cymelarsan	√	√	NP	NP	√	33,57,52
STIB 900	T. b. rhodesiense	+++	+++	NA	NA	√	unkn	√	unkn	unkn	56
STIB 900-M	T. b. rhodesiense	−	−	STIB 900	in vitro, Cymelarsan	√	unkn	NP	unkn	unkn	56
STIB 900-P	T. b. rhodesiense	−	+/−	STIB 900	in vitro, pentamidine	√	unkn	mutc	unkn	unkn	56

√, present; NP, not present; NA, not applicable; unkn, unknown; TGD, targeted gene deletion of the TbAT1 gene creating a tbat1 null line; MR or -M, resistance induced to melaminophenyl arsenicals (melarsoprol and/or Cymelarsan); -P, resistance induced to pentamidine.

^aSusceptibility to pentamidine or arsenical drugs is indicated on a relative scale as highly sensitive (+++) ranging to highly resistant to pentamidine or melaminophenyl arsenicals (−−−).

^bThe clone used in this paper is not tsetse transmissible but other clones of s427 have been shown to infect tsetse flies.³⁴

^cBernhard et al.⁵⁶ reported that STIB 900-P contained a wild-type TbAT1 gene; later analysis revealed that in fact the TbAT1 open reading frame contains one coding mutation (G1288C, leading to Gly⁴³⁰ → Arg).