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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Immunol. 2013 Sep 6;191(7):3858–3866. doi: 10.4049/jimmunol.1202999

Figure 1. Proteolysis of complement component C3 by kallikrein-related peptidases and trypsin.

Figure 1

The concentration-dependent KLK14-mediated generation of C3b-like fragments from degradation of C3 is followed (A), in comparison to the proteolytic processing of C3 by KLKs 5, 6, and trypsin (B–D) at the indicated enzyme to C3 ratios (w/w). Samples were run on 7.5% SDS-PAGE gels under reducing and denaturing conditions, and visualized with Coomassie staining. The C3 α- and β-chains are indicated with arrows, while the α΄-chain is indicated with black arrowheads. Lower molecular weight C3 fragments are indicated with grey arrowheads.