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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Dev Biol. 2013 Nov 21;386(1):165–180. doi: 10.1016/j.ydbio.2013.11.006

Fig. 10. Buffering of [Ca]i did not reduce PA-induced PLC activation.

Fig. 10

Xenopus eggs were treated with BAPTA-AM (250 μM, 1 h), which buffers [Ca]i and blocked the calcium-dependent fertilization events such as gravitational rotation and development, or carrier pluronic F-127/DMSO, did not significantly alter IP3 mass from that in control eggs. dPA (400 μM, 10 minutes) elevated IP3 mass and this elevation was not reduced when elevation of [Ca]i was prevented with prior treatment with BAPTA-AM.