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. Author manuscript; available in PMC: 2015 Feb 1.

Published in final edited form as: Dev Biol. 2013 Nov 21;386(1):165–180. doi: 10.1016/j.ydbio.2013.11.006

Fig. 11 — In the top row, dPA (400 μM) was added to albino Xenopus eggs preinjected with Fluo-4. In the middle row, 100 μM IP3 receptor blocker 2APB (100 μM) was added before 400 μM dPA and this eliminated [Ca]_i release. In the bottom row, 100 μM 2APB pretreatment before addition of the calcium ionophore ionomycin (10 μM) did not reduce [Ca]_i release. (B) Summarizing multiple experiments, 2APB reduced [Ca]_i release by dPA but not that released by ionomycin. (C) As measured by the time required to reach half maximal release, 2APB delayed dPA-induced elevation of [Ca]_i (but not that by ionomycin). “NR” represents no measurable [Ca]_i release by dPA.

Fig. 11 — In the top row, dPA (400 μM) was added to albino Xenopus eggs preinjected with Fluo-4. In the middle row, 100 μM IP3 receptor blocker 2APB (100 μM) was added before 400 μM dPA and this eliminated [Ca]_i release. In the bottom row, 100 μM 2APB pretreatment before addition of the calcium ionophore ionomycin (10 μM) did not reduce [Ca]_i release. (B) Summarizing multiple experiments, 2APB reduced [Ca]_i release by dPA but not that released by ionomycin. (C) As measured by the time required to reach half maximal release, 2APB delayed dPA-induced elevation of [Ca]_i (but not that by ionomycin). “NR” represents no measurable [Ca]_i release by dPA.