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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Dev Biol. 2013 Nov 21;386(1):165–180. doi: 10.1016/j.ydbio.2013.11.006

Fig. 4. 1-Butanol, but not 2-butanol, inhibited the Src and PLC activation in Xenopus fertilization.

Fig. 4

(A) Src activation was detected by Western blotting for phosphoSrc. At 2 minutes postinsemination, fertilization (“FERT” group; n=4; striped column) was associated with a 43% increase in phosphoSrc (P< 0.002). After a 1 hour preincubation, increasing levels of 1-butanol (filled bars) decreased the level of Src activation at 2 minutes, whereas 2-butanol treatment (grey bars) had no effect or increased phosphoSrc (asterisks denote P< 0.01). Attached at the bottom of panel A, total Src does not change with butanol treatment. (B) Sample Western blotting image of phosphoSrc (59 kDa) with a LI-COR Odyssey infrared system. Gel standards were: 250, 150, 100, 75, 50, 37, and 25 kDa. (C) As compared eggs alone (“EGGS”), IP3 mass increased 5 minutes after insemination (“FERT”) (n=4 for all groups) (asterisks denote P<0.05). The other two inseminated groups were preincubated (1 h) with 0.75% 1-butanol (“1-BUT+FERT”) or 2-butanol (“2-BUT+FERT”). 1-Butanol treatment decreased the IP3 elevation as compared with that of the control fertilization (P<0.0006) and 2-butanol treated (P<0.03). 2-Butanol treatment did not lower the IP3 peaks from control fertilization.