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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Dev Biol. 2013 Nov 21;386(1):165–180. doi: 10.1016/j.ydbio.2013.11.006

Fig. 5. PLD inhibitor FIPI inhibited fertilization events.

Fig. 5

(A) PA mass was measured before fertilization (EGG; n=5) and 4 min after insemination (FERT; n=7), and, in the third group (FIPI+FERT; n=8), 5 μM FIPI was added for 30 minutes before insemination. PA mass elevated at fertilization (P< 0.003) but FIPI inhibited this increase (P< 0.226). (B) PhosphoSrc levels increase from untreated eggs (time zero) at 2–3 minutes after insemination (shaded bars), whereas pretreatment with 10 μM FIPI (striped bars)(30 minutes) inhibited this increase (as compared to time zero, one asterisk denotes P < 0.03 whereas two signify P < 0.0001). (C) FIPI delayed gravitational rotation (a measure of cortical granule exocytosis) after insemination. Eggs (20 per group) were preincubated (30 minutes) with increasing concentrations of FIPI (open triangle: control fertilization; closed point: 1 μM FIPI; open circle: 10 μM FIPI).