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. Author manuscript; available in PMC: 2015 Feb 1.

Published in final edited form as: Dev Biol. 2013 Nov 21;386(1):165–180. doi: 10.1016/j.ydbio.2013.11.006

Fig. 6 — (A) The top row shows the [Ca]_i wave after insemination of albino Xenopus eggs, whereas the second and third rows show the [Ca]_i in the presence of the PLD inhibitors FIPI (10 μM) or 1-Butanol (0.75%). The bottom row shows cells treated with the control 2-Butanol (0.75%). The time for the determination is noted under each image (note the variance), and the highest [Ca]_i is represented by red, next by green, and blue represents the lowest [Ca]_i. (B) Pixels of [Ca]_i images from multiple experiments (numbers in the columns represent different cells) were summarized to show that (top) FIPI slowed the initiation of the sperm-induced calcium wave, and (bottom) reduced the size of the [Ca]_i increase (measured by recording relative changes from basal to peak fluorescence). The carrier for FIPI (1.3% DMSO) did not affect either time to wave initiation or maximal [Ca]_i. (C) As compared to controls, 1-Butanol delayed the wave initiation and reduced the size of the [Ca]_i increase. 2-Butanol had no effect. One asterisk denotes a P < 0.03 whereas two signify P < 0.0001.

Fig. 6 — (A) The top row shows the [Ca]_i wave after insemination of albino Xenopus eggs, whereas the second and third rows show the [Ca]_i in the presence of the PLD inhibitors FIPI (10 μM) or 1-Butanol (0.75%). The bottom row shows cells treated with the control 2-Butanol (0.75%). The time for the determination is noted under each image (note the variance), and the highest [Ca]_i is represented by red, next by green, and blue represents the lowest [Ca]_i. (B) Pixels of [Ca]_i images from multiple experiments (numbers in the columns represent different cells) were summarized to show that (top) FIPI slowed the initiation of the sperm-induced calcium wave, and (bottom) reduced the size of the [Ca]_i increase (measured by recording relative changes from basal to peak fluorescence). The carrier for FIPI (1.3% DMSO) did not affect either time to wave initiation or maximal [Ca]_i. (C) As compared to controls, 1-Butanol delayed the wave initiation and reduced the size of the [Ca]_i increase. 2-Butanol had no effect. One asterisk denotes a P < 0.03 whereas two signify P < 0.0001.